Frustration and Its Consequences

My friend Deb really frustrates me with her illness issues. Deb and I graduated from high school together and have remained close friends throughout the years. About five years ago, doctors diagnosed her with fibromyalgia and rheumatoid arthritis. Since then, her behavior has changed to somebody different, which actually frustrates me.Advertising We will write a custom essay sample on Frustration and Its Consequences specifically for you for only $16.05 $11/page Learn More I however, sometimes feel that Deb’s behavior does not come from her illness; it has become a mental problem, which frustrates and worries me. My thesis in this essay is that Deb’s behavior is no longer a result of illness but mindset. Based on her condition, I will demonstrate how her mentality has changed her behavior. Deb is always reflecting on the illness. While in high school, Deb was a bubbly, outgoing and a fun person; since the diagnosis however, she has changed from that kind of a person to someone who complains constantly of some illness. Even at times when she is not suffering from any pain, she complains of how she suffered the previous night or how she is likely to suffer the coming night. Her plans and thoughts revolve around the illness and this ends up frustrating all our plans. If we have made plans to do something, inevitably and regrettably a day or two before, she will call and cancel due to illness. The illness of other people reminds Deb of her sickness even when she is not suffering from any pain. My little knowledge on fibromyalgia informs me that people with this condition experience pain periodically, not as frequent as Deb insinuates. What frustrates me further is that when other people note to have a cold, sore back or anything, she will change the subject to her condition and something worse. Why should the situation of other people worsen her ill condition? I attribute this to her mindset. Looking at her physically, ev en when she is not suffering, she seems disturbed. She is always concerned about pills and her doctor. Whenever we are moving out, she has to carry her pills. This has set her mind such that she carries the pills even when she is not going far from the house. She always needs a pain pill for this or need to go to the doctor for that, and honestly this behavior worries and frustrates me.Advertising Looking for essay on psychology? Let's see if we can help you! Get your first paper with 15% OFF Learn More While sometimes I can justify her need for the pills, her carrying the pills most of the times is habitual. I am afraid that she is becoming addicted to being sick and the pills. In fact, she is ever discussing her encounters with the doctor, which frustrates me. I love her and just want her to get back to the person I initially knew. I am ever in contemplation on how to help her. Sometimes I think of consulting a psychiatrist for her situation but since she is so engro ssed with the fibromyalgia doctor, I find it difficult to convince her. In fact, it might cause more damage to her condition. I am therefore frustrated and I do not know how to help her. Her addiction to being sick, pills, discussing about her doctor and her illness really disturbs me. We no longer share the moments we used to; no outing or fun any more. Her illness has considerably affected her and her behavior is no longer a result of the illness but the mindset. In life, things are formed twice; in the mind and in reality (physically). Therefore, it appears Deb has formed a sickness in her mind and that’s what manifests in the physical. This essay on Frustration and Its Consequences was written and submitted by user Ada O. to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

drama improvisation essays

drama improvisation essays processes for the Elizabethan period In this essay based on improvisation, and the process that we undertook in the play building process I will describe the processes and stages that we went through but first, the main period of time that inspired our drama play was Elizabethan, theater during the Elizabethan times was often based around the lives and deaths of monarchs (kings and queens). Our play relates to the conventions of theater at the time because it was based around monarchs. The central purpose of this play was to point out the amount of superstition and envy that was often in the royal family but never seen by the public, as well as to entertain and educate the viewing audience about the Elizabethan times and what the Elizabethan plays were about and the sort of characters that were involved in these sort of plays: witches, kings, queens, princesses, soldiers, lady in waiting, and princes. As well as any other characters that may be required. The characters of out play are representative of characters of the sixteenth century because the witch planted a seed of ambition in the princess and the king goes off to battle, and the queen receives a report that the king is missing in action and presumed dead. The princess thinks that she should be the one to assume the throne because she is older than her brother but, when her brother is crowned heir to the throne the infuriated princess goes to the witch for a prediction of the future. This is where the witch plants the seed of ambition; this is an example of representation of characters of the sixteenth century. The plot of our drama performance was a reminiscent of the sixteenth century because there are witches, but the main similarity between our play and a drama performance of the sixteenth century is that in the sixteenth century drama a monarch was usually killed, in our play a monarch was also killed, the monarch tha ...

COMMONWEALTH of Pennsylvania, v Paul David CREWS Research Paper

COMMONWEALTH of Pennsylvania, v Paul David CREWS - Research Paper Example The case study of commonwealth of Pennsylvania vs. Paul David Crew is among the most valuable perspective pertaining to the use of DNA evidence in solving crime. The sensitive treatment of victims in DNA cases is essential across the entire criminal justice system; first beginning with the arrival of the responders at the crime scene and then continuing until long after suspects are convicted. All types of victims especially sexual assault victims should be subjected to fair and sensitive treatment with respect and dignity, especially during the collection of biological evidence from a wider range of crime scenes, which is now beginning to regularly occur in the United States. Problems regarding the security and privacy of a victim’s information in DNA cases are a major concern. The Victims’ DNA profiles are characteristically entered into databases which cause many victims to worry about the privacy of this information, whether it can be accessed by the perpetrators th rough the local databases or the internet. There is also concern about whether this technology can be used against victims who may have committed other crimes, which can possibly result in a decrease in reporting (PLJ, 2012). In this research paper, I chose the case of Commonwealth of Pennsylvania versus Paul David Crews in which many unique talents pertaining DNA evidence were involved in prosecuting the culprit. I am going to study facets of this case which I deem necessary to mention for the purpose of this research. Here, there is a combination of professional unique talents, the respect, their dignity and strive to foster their commitment to excellence in their work that led to justice. Unity and diversity of people on the free exchange of ideas, on learning, living and working harmoniously is truly the pillar of a community’s strength in society as we shall observe in this paper. For the purpose of grasping the whole concept of the case, I will briefly touch on other fa cets of the case other than those pertaining DNA evaluation. It is the professional unbiased performance of each that slowly but surely led to justice (PLJ, 2012). Commonwealth Of Pennsylvania, V Paul David Crews In the morning of September 13, 1990, two hikers, Geoffrey Hood and Molly LaRue, were murdered .The woman tied, raped, and stabbed, resulting to her death. Her boyfriend, shot three times. The suspect Paul David Crews was arrested week later and subsequently charged with the murders. There were a number of witnesses who presented themselves to testify. The first witnesses testified seeing the suspect visit a library seeking a map of the terrain where the couple was murdered while other witnesses testified the suspect seeking directions of the trail that coincidentally was the same with those of the murdered couple. These witnesses openly shared their knowledge to the relevant authorities. Some even vividly testified of seeing the suspect wearing the hiking gear belonging to the male victim along with other objects. A ballistics expert also testified that the handgun possessed by the suspect upon his arrest was the murder weapon which. An FBI DNA expert, after caring out his unique duty came to a conclusion thus testifying that the suspects DNA patterns matched with those obtained from semen samples the female victims vagina. The jury which comprises of carefully selected individuals of varying personalities and background

How newspapers will use social media and internet to expand readership Term Paper - 1

How newspapers will use social media and internet to expand readership in the future - Term Paper Example avvy world thus, the social media is strong enough to take over the traditional forms of transmission of news like newspapers, radio, television etc due to faster and better communication among people, rapidity of spread of news, an increase in readership, as well as a growing need to adapt and become environment friendly. Newspapers and radio channels are far behind than television today in terms of the spread of news for the sole reason that with the click of a channel or press of a button one can both see or hear the news on the go, as and when it takes place. Newspapers, even though they provide the audience with a chance to spend time reading and enhancing their knowledge, arrive a day later, by which time the person may already be well versed with the news. Newspapers however have a tangible form that many people are attached to and not willing to give up. Yet, it must be understood that newspapers can make use of the social media in order to expand their readership in the future as more and more people are logged onto the internet these days. Students, workers as well as professionals and home makers – almost everyone and anyone have an account on a social networking website. Thus in order to tap the potential, newspapers can exploit these platforms and display their news banner there, a ttracting readers onto their websites and thus help them in enriching themselves by displaying their news. (Rani Malla) Today, a number of newspapers have online editions, like Wall Street Journal and New York Times to name a few; these newspapers put up everything and more on their online versions, where people may subscribe and get hourly updates on the world’s events. Newspapers can also take over social networking websites to attract more readers by creating their pages and groups and involving forums for communication over the news among various people. Moreover, blogs and online journals are encouraged by newspaper reporters and writers in order to increase

Anti-Cancer Drug Screening in Vitro Essay Example for Free

Anti-Cancer Drug Screening in Vitro Essay Introduction: The incidences of cancer remain high despite advances in our understanding of cancer. Cancer is a class of diseases characterized by out of control cell growth. Normal cells are constantly subject to signals that control whether the cell should divide, differentiate into another cell or die. Cancer cells develop a degree of independence from these signals, which results in uncontrolled growth and proliferation. If this proliferation is allowed to continue and spread, it can be fatal (1). Almost 90% of cancer-related deaths are due to metastasis – the complex process of tumor spread through the lymphatic system or bloodstream. The emergence of genomic technologies holds therapeutic potential for personalized cancer management. Personalized cancer management combines standard chemotherapy and radiation treatments with genomic profiling and in vitro cell proliferation studies. Individualized genomic profiling allows the researcher to identify specific genes that contribute to unregulated cellular mechanisms that normally control cellular growth. By determining the molecular profile of a specific cancer, suitable treatment can be considered that target those gene products (2). Cultured cancer cells have the capacity to dramatically exceed normal doubling times to almost indefinite levels, unlike normal cells. HeLa cells are a great example of this. One of the most widely used continuous cell lines in research is the HeLa cell line, which was derived in 1951 from Henrietta Lacks, a cervical caner patient in 1951. These cells continue to grow and proliferate in hundreds of laboratories across the world to this day. These cancer cells have been called ‘Immortal’ as they have bypassed the senescence regulators within the cell and acquired the capacity for unlimited division. Measurement of cell viability and proliferation forms the basis for numerous in vitro assays of a cell population’s response to external factors. The reduction of tetrazolium salts is now widely accepted as a reliable way to examine cell p roliferation. MTT viability assays is based on the ability of a mitochondrial dehydrogenase enzyme from viable cells to cleave the tetrazolium rings of the pale yellow MTT and form a dark blue formazan crystals, which is largely impermeable to cell membranes, thus resulting in its accumulation within healthy cells. The resulting  intracellular purple formazan can be solubilized and quantified by a spectrometer and is then subject to examination to evaluate cell viability. These measurements can be used to evaluate the effectiveness of certain treatments to cells. These anti-cancer drugs in vitro allows drugs to be tested against live cells which helps determine drug effectiveness and side effects. Cell proliferation, also known as cell growth, cell division, or cell replication is the basic process through which cells form new cell. Cell proliferation is the increase in cell number as a result of cell division and growth. The quantification of cellular growth, including proliferation and viability, has become an essential tool in any laboratory working on cell-based studies. These techniques enable the optimization of cell culture conditions, and the determination of growth factor and cytokine activity. Even more importantly, the efficiency of therapeutic agents in drug screening, the cytostatic potential of anticancer compounds in toxicology testing, and cell-mediated toxicity can be assessed when quantifying cell growth (3). This practical is designed to evaluate the sensitivity and response of putative anti-cancer drugs using a modified anti-proliferative drug assay. Using a modified MTT drug assay, the sensitivity and response of anti-cancer drugs can be determined. In this blind trial, three unlabeled drugs are tested to discover their properties. The trial of these drugs was carried as a blind trial to insure that the results obtained with unbiased. Results In this practical, we were given three drugs. One of the drugs had no known anti-cancer properties; one was a known chemotherapeutic agent and the third was a mystery drug with putative anti-cancer properties. The HeLa cells were also treated with a no-drug, medium-only control. The objective of the study was to identify the nature of the unlabeled drugs. The identities of these three drugs were unknown in order to make the experiment a blind trial. Doing the study as a blind trial allowed any bias data to be avoided in data interpretation (2). Calculations: From the raw data obtained column 0 acts as the control, this column contains the HeLa cells but no drug, and is used to see if the unknown drugs A,B and  C have an effect on the viability of HeLa cells. The last row on the 96 well plates contained no HeLa cells and acted as a reference to observe whether column 0 contained living cells. When the absorbance value for column 0 was similar to the no cell value, that data set would be ignored, as this would indicate no cells were present in column 0 which would have been invalid as column 0 contained medium only. This method was applied when choosing appropriate data sets for data interpretation. Discussion: In this study we were given three drugs. One of the drugs had no known anti-cancer properties, one had a known chemotherapeutic agent and the third was a mystery drug with putative anti-cancer properties. The objective of this blind trial study was to identify the nature of these unlabeled drugs.  By observing the overall averages from the results (Table 1 Figure 1) we can conclude that drug A had the highest percentage cell viability out of the three drugs. The percentage value of A is similar to the percentage value of the control (0). The control consisted of a medium-only solution and not meant to effect the cell viability of HeLa cells at all, this implies that Drug A has no anti-cancer properties and has little effect on the HeLa cells viability. From this data we can conclude that Drug A was the drug with no known anti-cancer properties (negative control). By observing the percentage cell viability of drug B, we can conclude that drug B had the smallest cell viability value that was close to 0%. Small percentage viability indicates that there is a reduction in cell proliferation occurring in the HeLa cell line, however 0% cell viability would indicate that there are no viable cells in the well, meaning there was a complete reduction in cell proliferation. Lack of cell proliferation means an absence of mitochondrial succinic dehydrogenase activity to metabolize MTT into its results purple formazan, producing a lower absorbance value (4). From this we can conclude that drug B has anti-cancer properties as it had a large negative effect on HeLa cell viability. By observing drug C, we can determine that drug C also has a relatively low percentage cell viability value. This would indicate that drug C, like drug B, has a negative effect on cell viability, meaning it causes a reduction in cell proliferation in HeLa cells. From this we can conclude that Drug C also had anti-cancer properties as it had a negative effect on HeLa cell viability. At this point, two drugs have yet to be identified, Drug B and Drug C. Both Drug B and Drug C have anti-cancer properties, shown by their low cell viabilities values. A positive control will be a drug that will give a large cell viability percentage. From this, we would conclude that Drug B was the positive control with a chemotherapeutic agent leaving Drug C to be the mystery drug. However we cannot accept this without actually knowing what the mystery drugs anti-cancer properties are. The mystery drug could have stronger anti-cancer properties than the positive control. In this scenario, Drug B would be the mystery drug as it observed the lowest cell viability, making it the most effective against cancer cells. For this reason, we cannot accurately identify drugs B and C without more information about the mystery drug. However if we accept that the mystery drug has a smaller  effect on cancer cells than the positive control, we would then conclude that Drug B is the positive control and Drug C is the mystery drug. If we observe the overall data used to calculate the average cell viability percentages for each drug, we can see there are values higher than 100% and values lower than 0% (negative values). Values higher than 100% would indicate that there are more viable cells present in drug A compared to our control. Since we know drug A has no effect on the cancer cells, we would expect the cell viability of drug A to be similar to the control. These anomalies could be due to cell proliferation in the drug A column, meaning the HeLa cells grew and divided to create new cells, meaning the number of cells in column A increased, resulting in a higher absorbance value, due to the increase in MTT reduction. A higher absorbance value in the no cell row compared to the drug B column results in a negative value. Absorbance values that are lower than the control cells indicate a reduction in the rate of cell proliferation. Conversely a higher absorbance rate indicates an increase in cell proliferation. Values lower than 0% would indicate that there are fewer cells present in the drug B wells compared to the no cell wells. This could be due to human error where cells where accidently transferred due to poor lab technique, or been caused by a high absorbency of the buffer used in the no cell row. In this study, the reduction of the MMT is used to estimate cell viability and proliferation. However recent studies have shown that superoxide can also reduce tetrazolium salts, such as MTT. Therefore studies investigating the cytological effect of HeLa cells may encounter misleading results when using MTT to measure viability proliferation. This is because MTT assays may yield inaccurate results due to the increase in superoxide formation in cultured HeLa cells (7). This kind of limitation may have played apart in this study and could have caused our abnormal values discussed in the paragraph above. To overcome this limitation, we could use different techniques to measure cell viability. One way of assessing cytotoxicity is by cell integrity. Compounds that have cytotoxic effects, such as the drugs we are investigating, often compromise cell membrane integrity. An example of this method is a Tryphan Blue exclusion test (10). This exclusion test can be used to determine the number of viable cells present in a cell suspension. It is based on the principle that viable cells have intact cell membranes that are impermeable to dyes,  such a trypan blue, whereas dead cells do not. In this test, a cell suspension is mixed with the tryphan blue dye and then visually examined to determine whether cells take up or exclude the dye (11). A viable cell will have a clear cytoplasm whereas a dead cell will have a blue cytoplasm. Tryphan blue is not affected by superoxide formation so will yield more accurate results than the MTT assay. The problems that arise from dye exclusion tests is that they are operator depend and are subject to human error. Another way of assessing cell viability via membrane integrity is by using fluorescent DNA binding dyes such as SYBR Green I (10). SYBR Green I is a fluorescent dye used as a nucleic acid dye. SYBR Green I binds to DNA and the resulting DNA-dye-complex absorbs blue light and emits green light (9). This is based on the principle that an increase in cell proliferation will cause an increase of DNA in the cell suspension. More SYBR Green 1 will bind to the DNA and more green light will be observed under blue light.

Hawthorne: An Inescapable Burden :: essays research papers fc

An Inescapable Burden   Ã‚  Ã‚  Ã‚  Ã‚   A young boy’s parents did not get the opportunity to attend college and they divorced when he was only seven. His father was addicted to crack cocaine and his mother began to sell herself as a source of income. He was tossed back and forth between the two houses and used as a way for the two parents to get back at each other. As a result, this boy grew up to be a criminal and was thrown in jail for selling drugs for his father. This life of crime and debauchery was all that he knew. He constantly paid for the mistakes of his parents and his parents’ parents. This is a common event that has taken place in human society throughout time. Nathaniel Hawthorne recognized the fact that people have the burden of constantly dealing with the shortcomings and lack of opportunity that come with many mistakes that parents make. This theme and realization of Hawthorne’s is a prevalent theme in many of his stories. This theme and idea that one must pay for the mistakes of his o r her forefathers is displayed not only in Hawthorne’s own history but in his stories â€Å"Dr. Rappacini’s Daughter,† â€Å"The Birthmark,† and â€Å"Young Goodman Brown† as well.   Ã‚  Ã‚  Ã‚  Ã‚  Hawthorne had a family history which he was personally ashamed of. He was born in Salem Massachusetts in 1804 with the emotional burden of having a great-great grandfather that was the judge that presided over the Salem witch trials (Gollin 1). This man wrongfully convicted many women that were put to death as a result of his conviction. The blood of these women was on his hands and it was probably the thought in Hawthorne‘s mind that his ancestor did the devil’s work . This family history is most prominently displayed in his story â€Å"Young Goodman Brown† as Goodman faces similar internal conflict during his journey (Gray 90). Hawthorne thought that his sea captain father paid for these mistakes as he was killed on a voyage when Hawthorne was four years old (â€Å"Nathaniel Hawthorne† 259). Hawthorne could have conceived this idea directly from God’s own word. The Bible claims that God will inflict â€Å"punishment for their f ather’s wickedness on the children of those who hate [him] down to the third and fourth generation† (Exodus 20.5). Hawthorne’s own personal family history imbedded this theme in his own theologies.   Ã‚  Ã‚  Ã‚  Ã‚  This theme is displayed in Hawthorne’s short story â€Å"Dr.

Imperialism and Manhood Essay

Study question 1. 2. 3. To Roosevelt, the idea of race suicide was closely interlinked to manhood. Not only would the American men face competition from immigrant workers and be unable to provide for more children: thus leading to an excess of inferior immigrants but the American man would also be related to the impotent American man, unable to create more children. This would lead to the race’s elimination Race and Immigration 1. It allowed individuals to distinguish and separate themselves from immigrants thus creating a barrier that showed them as undesirable. 2. Certain policies and laws were created to separate the Chinese from other groups: Chinese Exclusion Act. 3. This act set a precedent on the treatment of other foreigners that would enter America. Urban Politics and Culture 1. It is preferable to have a community that has a degree of refinement than to live in one that is full of poverty. 2. The wealth should be divided amongst the immediate family to a degree that does not surpass them into leading unfulfilling and lazy lives. The rest should be distributed for the benefit of society. 1. Having undergone poverty himself, Riles shows stereotypes but presents them in an entertaining way to the audience. Also he mentions the roles these different cultures play in merging with the American way of life. 2. Riis and Carnegie are similar in their opinion that the poor exist and cannot be ignored but it is only the rich who can provide them with relief. Cultural Encounters in the Far West 1. The Bison went into extinction because of the cold and droughts bought in by the changing environment and human influence which resulted in colonial expansion that bought in new diseases and animals. 2. It was not the nomads alone who hunted the bison. They sold the animals to the Euro-American invaders. 1. Blacks could not vote unless their grandfathers had not been voters before the Civil War, literacy tests had to be passed, fees was charged for voting and democrats (always white individuals) were the only ones allowed to vote. 2. The South contained individuals who had the deepest hatred for the blacks which led the lynching in this area to be the worst. 3. Separate schools, hospitals, accommodations and even entrances were given to the two groups. Often enough the blacks were provided no facilities by certain states. 4. They decided to test out the marginzalation itself by acts like the one committed by Homer A Plessy Jim Crow Museum of Racist Memorabilia Website 1. Minstrels would perform music with black faced performers in the quest to ridicule black people. Also caricatures were made which were printed in novels and plays. 2. Churches taught that blacks were inferior. Politicians gave speeches supporting segregation and writers also wrote against the black man.